Phytochemical Analysis and Anticancer Activity of Essential Oil from Myristica fragrans

Mary, Helen P.A and Tina, Ann Vargheese and Jeeja, Kumari J.J. and Abiramy, M.R (2012) Phytochemical Analysis and Anticancer Activity of Essential Oil from Myristica fragrans. International Journal of Current Pharmaceutical Review and Research, 2 (4). pp. 188-198. ISSN 0976-822X


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Volatile oil from the leaves of Myristica fragrans was isolated and characterized by Gas Chromatography- Mass Spectroscopy. Twenty constituents from 20 peaks were identified beta- pinene (22.69%), alpha-pinene (14.06%), alpha-thujen (13.93%) and p-menth 1-en-4-ol (10.53%) are as the major constituents and this leaf oil contains the minor constituents like camphene (3.92%), α-terpinene (3.42%), Limonene (9.56%) p-cymene (6.86%) Linalool (7.41%), beta myrcen (4.81%), alpha fellandrene (3.14%), 3-carene (3.54%), allyl catechol methylene (8.32%), myristicin (7.20%), elermicin (9.85%), beta ocimene (4.74%), gamma terpinene (4.75%), alpha terpinolene (4.38%), p-menth 2-en-1-ol (3.30%) and α-terpineol (6.00%). The antimicrobial activity of oil was tested against human and plant pathogenic bacteria and fungi. The oil showed significant inhibitory activity against the bacteria, Enterococcus faecalis (1.3cm), Lactococcus plantarum (0.9cm) and Proteus vulgaris (0.6cm) and the fungus Candida tropicalis (1.3cm), Candida albicans (0.8cm), Rhizomucor miehei (0.6cm) and Candida glabrata (0.6cm). No inhibitiory activity was observed against the bacteria Clostridium pertringens, Klebsiella Pneumoniae and Bacillus megaterium. There is no inhibitory activity of oil against the fungi, Aspergillus niger and Aspergillus fumigates. Using fluorescent stains localize cortex region, phloem fibres, oil ducts, phenol secreting cells and vascular cambial cells. Myristica fragrans showed 100% larvicide activity. The addition of various concentrations of essential oil of Myristica fragrans in the MCF-7 breast cancer cell line and A-357 epidermal skin cancer cell line showed cytotoxic activity.

Item Type: Article
Uncontrolled Keywords: Myristica fragrans, Antimicrobial Screening, GC/MS Analysis, Histochemistry, Anticancer, Larvicide.
Subjects: Q Science > Q Science (General)
Q Science > QD Chemistry
R Medicine > R Medicine (General)
R Medicine > RC Internal medicine
R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer)
Divisions: Faculty of Engineering, Science and Mathematics > School of Chemistry
Depositing User: Mr Tayo Okunlola
Date Deposited: 29 Jun 2016 08:44
Last Modified: 29 Jun 2016 08:44

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