High performance liquid chromatography (HPLC) fingerprinting and in vitro antioxidant activity of methanol leaf extract of napoleona vogelii (lecythidaceae)

Obafemi, T. O. and Afolabi, B.A and Falode, J. A. and Adeoye, A.O. and Afolabi, O.B. and Awe, J.O. and Fadaka, AO and Ochuko, O. and Jacdonmi, T and Boligon, A.A. and Athayde, M.L. (2016) High performance liquid chromatography (HPLC) fingerprinting and in vitro antioxidant activity of methanol leaf extract of napoleona vogelii (lecythidaceae). PharmacologyOnline, 1. pp. 109-119. ISSN 1827-8620

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Abstract

The use of plants for medicinal purposes has gained widespread acceptance over the years. The therapeutic activities of medicinal plants so used are not unconnected with their chemical composition. In the present study High-Performance Liquid Chromatography with Diode-Array Detection (HPLC-DAD) was used to quantify the flavonoids, phenolic acids and carotenoids present in the methanol leaf extract of Napoleona vogelii (lecythidaceae) (MNV). Phytochemical composition of the extract was evaluated. Standard protocols were also used to estimate the total phenols and total flavonoids content of the extract. Nitric oxide (NO), hydroxyl radical (OH- ), 2,2’-azinobis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS·+) and 2,2- diphenyl -1-picrylhydrazyl (DPPH) radicals scavenging activity, inhibition of lipid peroxidation and the ability of MNV to chelate ferrous ions as well its reductive potential were also evaluated. The presence of flavonoids, saponins, cardiac glycosides and terpenoids were confirmed in the extract. Our results show that Napoleona vogelii has excellent ABTS·+ and DPPH radicals scavenging activity. The extract was found to compare favourably with ascorbic acid in the inhibition of lipid peroxidation. We also observed that the extract chelated ferrous ions and inhibited NO to an appreciable level, while showing a moderate reducing power and inhibition of OH- . The inhibitory concentration (IC50) values for the various radical scavenging assays were DPPH - 187.10 μg/ml, NO - 145.05 μg/ml, OH- - 243.90 μg/ml and ABTS – 115.63 μg/ml. The HPLC-DAD analysis showed significant levels of flavonoids, phenolic acids and carotenoids. From the foregoing, we conclude that methanol extract of Napoleona vogelii could be considered relevant in the treatment modalities for a wide array of diseases because of the presence of several biologically active compounds which might be responsible for the observed antioxidant activities.

Item Type: Article
Uncontrolled Keywords: Flavonoids, phytochemical, peroxidation, hydroxyl radical, analysis
Subjects: R Medicine > R Medicine (General)
R Medicine > RB Pathology
R Medicine > RC Internal medicine
R Medicine > RL Dermatology
Divisions: Faculty of Medicine, Health and Life Sciences > School of Medicine
Depositing User: Mr Tayo Okunlola
Date Deposited: 11 Jul 2016 13:05
Last Modified: 11 Jul 2016 13:05
URI: http://eprints.abuad.edu.ng/id/eprint/1054

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