P53, Bax and Cathepsin D Dysregulation in Neurons Subjected to Cyanide Toxicity and Oxygen Deprivation

OGUNDELE, Olalekan Michael and ADEKEYE, Adeshina Oloruntoba and ADENIYI, Philip Adeyemi and OGEDENGBE, Oluwatosin Olalekan and ENYE, Linus Anderson and SAHEED, Sabiu and ANOSIKE, Victor Ikenna (2013) P53, Bax and Cathepsin D Dysregulation in Neurons Subjected to Cyanide Toxicity and Oxygen Deprivation. World J Life Sci. and Medical Research, 3 (1). pp. 1-6. ISSN 2249-0574

[img] Text
en_wjlsmr_vol_3_iss_1_pg_33_38.pdf - Published Version

Download (256kB)
[img] Text

Download (256kB)
Official URL: http://www.rrpjournals.org


Cyanide is a potent neurotoxin capable of potentiation NMDA R1 (N-methyl-D-aspartate receptor 1) a form of glutamate receptor that is calcium gated, thus causing excitotoxicity. It is also well established that the glutamate-glucose exchange is dependent on the activity of the Na + /K + ATPase pump, thus we examine the role of the Na + /K + pump in the metabolism of the neuron during cyanide toxicity. Six separate perfusion set up of the rat brain cortical tissues were made with ACSF (ACSF, ACSF+KCN, ACSF+KCN + pump blocker, ACSF + pump blocker). The tissues were perfused for duration of 180 minutes. The tissues were processed immunohistochemically using antibodies against p53,Bax and Cathepsin D (CD) to demonstrate disregulation of cell cycle proteins associated with the induced DNAbreakage as a result of cyanide toxicity. The pumpblockers (methyldigoxin and promethazine) induced excitotoxicity when used in culture, and amplified cyanide toxicity when combined with KCN. Cell death induced by toxicity of cyanide and the blockade of the Na/K ATPasepump has been seen to be complimentary in driving the toxicity effects that drives the cell into apoptosis.The tumor suppressor/apoptosis inducing factors p53 and Bax were over expressed while cathepsin was suppressed to show that the cells are apoptotic as against an increased cathepsin D level that would have implied senescence.

Item Type: Article
Uncontrolled Keywords: Neuron, hydrogen cyanide, oxygen, P53, Bax and Cathepsin D
Subjects: Q Science > QM Human anatomy
Divisions: Faculty of Medicine, Health and Life Sciences > School of Biological Sciences
Depositing User: Mr. Victor Sebiotimo
Date Deposited: 13 Mar 2019 14:14
Last Modified: 13 Mar 2019 14:14
URI: http://eprints.abuad.edu.ng/id/eprint/111

Actions (login required)

View Item View Item