Incidence of multiple antibiotic resistance among Salmonella spp. isolated from poultry droppings and cow dung in Ado-Ekiti metropolis

Oluyege, J.O. and Oyinloye, I.A. (2013) Incidence of multiple antibiotic resistance among Salmonella spp. isolated from poultry droppings and cow dung in Ado-Ekiti metropolis. Researcher, 5 (9). pp. 32-36. ISSN 1553-9865

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Abstract

Incidence of multiple antibiotic resistance has been observed among Salmonella species. The main objective of this study was to determine the multiple antibiotic resistance pattern in Salmonella isolated from poultry droppings and cow dung. Samples of poultry droppings and cow dung were randomly collected from poultry houses and abattoirs in Ado-Ekiti. Isolation was done using Salmonella-Shigella agar, after which the isolates were subjected to some biochemical tests. The isolates were also subjected to confirmatory test using Salmonella test kit. The confirmed Salmonella isolates were subjected to antibiotic susceptibility tests using antibiotic discs such as Pefloxacin (5µg), Ofloxacin (5µg), Ciprofloxacin (10µg), Norfloxacin (10µg), Amoxicillin (10µg), Nalidixic acid (30µg) and Nitrofurantoin (300µg) and their multiple antibiotics resistance (MAR) pattern were observed. Statistical analysis using student t-test was done to compare the mean resistance of Salmonella sp. isolated from different environment to the different antibiotics used. Eighty-seven isolates were recovered from the samples, forty six from cow dung and forty one from poultry droppings. When the isolates were subjected to confirmatory test using Salmonella test kit, sixty eight were found to be positive by showing agglutination within two minutes. The percentage resistance observed among Salmonella species isolated from cow dung and poultry droppings were amoxicillin (73.5%), nitrofurantoin (63.2%), nalidixic acid (60.3%), pefloxacin (52.9%), norfloxacin (36.8), ciprofloxacin (35.3%) and ofloxacin (20.9%). The highest resistance among the isolates was observed to amoxicillin and the least was to ofloxacin. The MAR patterns observed among the isolates include: PEF/OFX/AMX/CIP/NAL/NOR/NIT, PEF/AMX/NAL/NOR/NIT, PEF/AMX/NOR/NIT and CIP/NAL/NOR. Statistical analysis revealed that there was no significant difference in the resistance of Salmonella sp. from different sources to the different antibiotics used. It can be deduced from this study that those isolates showing multiple antibiotic resistance pose a threat to human population because if they find their way into the human population they can cause resistance to antibiotics in patients suffering from Salmonella infection.

Item Type: Article
Uncontrolled Keywords: Abbatoir, Cow dung, Multiple antibiotic resistance (MAR), Salmonella, Poultry droppings
Subjects: Q Science > Q Science (General)
Q Science > QL Zoology
Q Science > QR Microbiology
Q Science > QR Microbiology > QR355 Virology
R Medicine > R Medicine (General)
R Medicine > RA Public aspects of medicine
R Medicine > RA Public aspects of medicine > RA0421 Public health. Hygiene. Preventive Medicine
Divisions: Faculty of Medicine, Health and Life Sciences > School of Biological Sciences
Depositing User: Mr Tayo Okunlola
Date Deposited: 15 Aug 2016 14:23
Last Modified: 15 Aug 2016 14:23
URI: http://eprints.abuad.edu.ng/id/eprint/1178

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