Screenning of extracts ofHibiscus sabdariffa andAzardirachta indica forbioactive compounds

Ojezele, Matthew Obaineh and Agunbiade, Shadrach Oludare and Adeosun, Abiola Muhammad (2013) Screenning of extracts ofHibiscus sabdariffa andAzardirachta indica forbioactive compounds. International Journal of Traditional and Herbal Medicine, 1 (5). pp. 153-158. ISSN 2345-3249

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Abstract

International Journal of Traditional and Herbal Medicine. Vol., 1 (5),153-158, 2013Available online athttp://www.ijthmjournal.comISSN 2345-3249 ©2013 IJTHMScreenning of extracts ofHibiscus sabdariffa andAzardirachta indica forbioactive compoundsOjezele Matthew Obaineh1*,Agunbiade Shadrach Oludare1,Adeosun Abiola Muhammad11-Department of Biochemistry, Lead City University, Ibadan, Oyo State, Nigeria*Corresponding Author:Ojezele Matthew ObainehAbstractIt is important to screen secondary metabolites in plants as a major step towards theelucidation of their therapeutic mechanisms. This study focused on screening secondarymetabolites ofAzardirachta indica(neem) plant parts andHibiscus sabdariffa calyx extractsas a major step toward the elucidation of therapeutic potential. Standard biochemicalprocedures were used to analyze aqueous (hot and cold) and ethanol extracts of the plantparts for bioactive components. Tannin and alkaloids in neem were high in both bark (5.23-6.54/ mg/g; 12.6-19.9%) and leaf (5.19-17.31 mg/g; 7.3-53.2%); the highest value wasrecorded in the ethanol leaf extract (17.31mg/g; 53.2%). The highest yield of all the bioactivecomponents inH. sabdariffa was found in the hot water extract. Oxalate yield was highest inthe hot water extracts of all the plant parts. Extracts ofAzardirachta indica (leaf, root andbark) andHibiscus sabdariffa (calyx) contain useful bioactive components which may beresponsible for thefolklores and scientifically investigated beneficial medicinal effects.However, there may beneeded to be cautious in excessive consumption of hot waterextracts of the plants because of the presence of oxalate

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