Antimicrobial Activities of Different Honeys Sold in Ado-Ekiti on Bacteria Associated with Upper Respiratory Tract Infections

Oluboyo, Bernard Oluwapelumi and Akinfemiwa, Morayo and Ayuba, Sunday Buru and Akele, Yomi Richard and Akinseye, Janet Funmilayo and Adewumi, Ajoke Funmi (2017) Antimicrobial Activities of Different Honeys Sold in Ado-Ekiti on Bacteria Associated with Upper Respiratory Tract Infections. International Journal of Current Microbiology and Applied Sciences, 6 (2). pp. 1-11. ISSN 2319-7706

[img] Text
OluboyoBernardOluwapelumietal-2.pdf - Published Version

Download (328kB)
Official URL:


Honey has been used for various purposes including treatment of some diseases locally especially in wounds and upper respiratory tract infections (URTIs). There is paucity of information on the scientific basis for the use of honey in the treatment of uppe r respiratory tract infections caused by bacteria. This study was carried out to determine antibacterial activities of different brands of honey marketed in Ado-Ekiti and relate with those of some orthodox antibiotics used in the treatment of URTIs. The an timicrobial activities of the honeys were assessed against Staphylococcus aureus, Pseudomonas aeruginosa and Klebsiella pneumoniae using agar well diffusion technique. Broth dilution method was used to determine the minimum inhibitory concentration (MIC) of the honey against the bacteria. Screening concentrations of neat honey and 80% honey inhibited Staphylococcus aureus and Klebsiella pneumoniae with zone of inhibition ranging between 2-10 mm. Pseudomonas aeruginosa was resistant to all concentrations of honeys except for one honey (Dr Bee) that inhibited the organism at 100% concentration. Orthodox antibiotics showed significantly higher mean zone of inhibition in relation to honey (P < 0.001). The sensitive organisms were inhibited in dose related manner. The mean MIC of the different honey brands was generally at 31.25% against Staphylococcus aureus while concentration of 50% showed bactericidal effect on S. aureus. Klebsiella pneumoniae was inhibited at mean concentration of 31.5% and bactericidal effect was observed against the organism at 40% mean concentration.

Item Type: Article
Uncontrolled Keywords: Mounyr, B., S. Moulay, Saad, K. I. 2016. Methods for in vitro evaluating antimicrobial activity: a review. Journal of Pharmaceutical analysis, 6 (2): 71-79 Mundo, M. A., O. I. Padilla-Zakour, and Worobo R. W. 2004. Growth inhibition of foodborne pathogens and food spoilage organisms by select raw honeys. Int. J Food Microbiol. 97, 1–8. Olawuyi, A., F. Fasina, S. Ardzard, G. Kano, G. Agada, and Gado, D. 2010. Antimicrobial activities of honey from different locations on Gram negative and positive organisms. Niger. Vert. J., 31 (2): 103-114. Olusegun, O. 2013. Urban expansion and urban land use in Ado Ekiti, Nigeria. American Journal of Research communication, 1 (2): 128-139 Nzeako, B. and Hamdi, J. 2000. Antimicrobial potential of honey on some microbial isolates. S. Q. U. J. Sci. Res. (Med. Sci.). 2, 75-79. Paul, I. M., J. Beiler, A. McMonagle, M. L. Shaffer, L. Duda, and Berlin C. J. Jr. 2007. Effects of honey, dextromethorphan, and no treatment on nocturnal cough and sleep quality for coughing children and their parents. Arch Pediartr Adelesc Med., 161 (12):1140-1146 Poole, M. D. and Portugal, L. G. 2005. Treatment of Rhinosinusitis in the Outpatient Setting. Am J Med., 118, 45S-50S. Roland, N., E. Alertia, M. Christy, T. JaneFrancis, M. Lucy, and Henry N. 2007. In vitro antimicrobial activity of selected honeys on clinical isolates of Helicobacter pylori. African Health Sci., 4, 228-232 Subrahmanyam, M. 1991. Topical Application of Honey in the Treatment of Burns. British J. Surgery, 78, 497-498. Willix, D., P. Molan, and Harfoot, C. 1992. A composition of the sensitivity of wound infecting species of bacteria to the antimicrobial activity of Manuka honey and other honey. J Appl Bacteriol. 73, 388–394. Wood, S. and Washington, J. 1995. Antimicrobial susceptibility test; dilution and disk diffusion methods. Manual of Clinical Microbiology, 6 th edition. American Society for Microbiology, Washington, D.C., pp. 1327-1332. Worldatlas, 2016. http://www.worldatlas. com/af/ng/ek/where-is-ado-ekiti.html Assessed 16th December, 2016 Zaghloul, A. A., H. H. El Shattawy, A. A. Kassem, E. A. Ibrahim, I. K. Reddy and Khan, M. 2001. Honey, a Prospective Antibiotic: Extraction, Formulation, and Stability. Pharmacies, 56, 643-647.
Subjects: Q Science > QR Microbiology
Divisions: Faculty of Medicine, Health and Life Sciences > School of Medicine
Depositing User: Mr. Victor Sebiotimo
Date Deposited: 18 Mar 2019 14:19
Last Modified: 18 Mar 2019 14:19

Actions (login required)

View Item View Item