Assessment of the Microbiological Quality and Efficacy of Two Common Disinfectants Used in Hospital Laboratory

Enitan, Seyi Samson and Okeleke, Ochei John and Awharentomah, Digban Kester and Akele, Yomi Richard and Nwankwo, Kasie Josephine and Arisi, Chinedu Paul Assessment of the Microbiological Quality and Efficacy of Two Common Disinfectants Used in Hospital Laboratory. Advances in Biomedical Sciences, 2 (6). pp. 31-43.

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Abstract

The present study assessed the microbiological qual ity and efficacy of two common disinfectants (Jik a nd Lysol) used in a hospital laboratory. Sterility test using Nutrient Agar and Sabour Dextrose Agar plates incubated at 3 7°C and 25°C, respectively, were employed to detect the present o f potential bacterial and fungal contaminants in 3 new batches of stock disinfectants. Swabs of work-bench surfaces designa ted as Site 1, 2 and 3 were collected in triplicate at the end of each business day ( i.e, before disinfection) and also after disinfection wi th 30% Jik and 2.5% Lysol dilution and cultured in tubes containing 3 ml of Tryptic Soy Broth medium and 0.1 mL Neutralizer. Surface viable count was carried o ut to determine the bacterial population density of three sites pre-dis infection and post-disinfection. Colonies of bacter ia were identified by Gram- stain, motility test and routine biochemical tests. The efficacy of the disinfectants against each bac terial isolate at 10 min contact time was determined using the quantitative suspension test. The killing rate of the disinfecta nts was expressed by plotting the logarithms of surviving cells (CFU/mL) against exposure time (min) of the disinfectant. T he outcome of the study showed that the microbiological quality of the two disinfectants tested was satisfactory. Bacterial di stribution pre-disinfection include: Staphylococcus epidermidis , Enterococcus aerogenes, Proteus mirabilis, Bacillus subtilis, Pseudomonas aeruginosa and Klebsiellia pneumoniae ; while only B. subtilis, P. aeruginosa and K. pneumoniae were recovered post-disinfection. Lysol proved to be more potent than Jik at the dilution a nd contact time tested with a log reduction of bact erial population ≥5. S. epidermidis , E. aerogenes and P. mirabilis were completely killed by the two disinfectants wi thin the 10 minutes contact time. While, a population density of 1-2 log CFU/ml of B. subtilis, P. aeruginosa and K. pneumoniae still survived after 10 min exposure to Lysol and Jik. The outcome of this stud y further strengthening earlier works and underscor ed the need to periodically assess the microbiological quality and efficacy of disinfectants routinely supplied to th e laboratory to ensure proper control of infections by using right disinfe ctant in right concentration for a right contact ti me

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