Anti-nociceptive and anti-inflammatory activities of Tetracarpidium conophorum seed lectin

Osukoya, Olukemi Adetutu and Oladokun, Boniface Oladiran and Omisore, Omotayo Nusirat and Kuku, Adenike (2019) Anti-nociceptive and anti-inflammatory activities of Tetracarpidium conophorum seed lectin. Scientific African. pp. 1-9. ISSN 2468-2276

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The anti-nociceptive and anti-inflammatory activity of the lectin purified from Tetracarpidium conophorum seeds were investigated. The lectin was purified from the phosphate buffered saline crude extract of the seeds using gel filtration and affinity chromatography techniques. Hemagglutination assay and hapten-sugar inhibition test were carried out to ascertain the blood group and sugar specificities of the lectin respectively. Anti-nociceptive and anti-inflammatory activities of the lectin were evaluated (in-vivo) with standard models including formalin licking test, carrageenan-induced paw edema test and peritonitis model. A yield, purification fold and subunit molecular weight of 27%, 17 and 34 kDa respectively were obtained for the purified lectin from T. conophorum seed (TcSL). The lactose/galactose specific TcSL showed significant inhibition (p < 0.05) of nociception as measured by paw licking time upon pain induction by formalin and inflammation as measured by paw swollenness induced by sub-plantar injection of carrageenan. The lectin also significantly reduced carrageenan-induced leucocyte migration to the peritoneum in a dosedependent manner. The study concluded that TcSL showed anti-nociceptive and anti-inflammatory activities and thus poised to be a good bio-active peptide for the design of novel anti-inflammatory therapy

Item Type: Article
Uncontrolled Keywords: Nociception Inflammation Lectin seed Tetracarpidium conophorum
Subjects: Q Science > QD Chemistry
Divisions: Faculty of Engineering, Science and Mathematics > School of Chemistry
Depositing User: Mrs Ayomide Rasaq
Date Deposited: 30 Mar 2020 17:17
Last Modified: 30 Mar 2020 17:17

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