Effect of methanolic neem bark extract on the testicular parameters of adult sprague-dawley rats

Adeshina, Oloruntoba Adekeye and Adedayo, Damilare Adekomi and Oluwatosin, Olalekan Ogedengbe and Adedoyin, Mojeed Agbaje and Francis, Duru (2013) Effect of methanolic neem bark extract on the testicular parameters of adult sprague-dawley rats. Journal of Medicine and Medical Sciences, 4 (3). pp. 117-122.


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The experiment was conducted to determine the effect of methanolic neem bark extract (Azadirachta indica) on some reproductive parameters of male Sprague – Dawley rats. Twenty adult Sprague-Dawley rats were used weighing between 120 – 160g. They were randomly allocated to 4 groups with 5 animals in each group. Rats in groups A, B, and C received 100 mg/kg, 200 mg/kg and 400 mg/kg of methanolic neem bark extract respectively. Group D (control) was administered with distilled water daily. The duration of administration was for 56 days (56d). At the end of the experiments, the rats were sacrificed using chloroform. Testes were excised, weighed and fixed in Bouin’s fluid and were later processed for further histological examinations. Blood was collected through cardiac puncture, centrifuged and was assayed for serum testosterone analysis using the ELISA kit. Result on testicular histology showed a significant distortion of the testicular tubular epithelium in all the treated groups with a reduction in interstitial cell of Leydig. There was an insignificant increase in testicular weight in the treated groups when compared with the control group. Double tail sperm morphology and reduced sperm motility was observed in all the treated groups. Also the serum testosterone levels showed a significant decrease in all treated groups compared to the control (p<0.05). The results of the study suggested that Azadirachta indica bark extract has a deleterious effect on semen and the studied reproductive parameters in male rats.

Item Type: Article
Uncontrolled Keywords: Azadirachta indica, Bouin’s fluid, ELISA test kit, Leydig, Chloroform.
Subjects: R Medicine > R Medicine (General)
R Medicine > RM Therapeutics. Pharmacology
R Medicine > RS Pharmacy and materia medica
Divisions: Faculty of Medicine, Health and Life Sciences > School of Medicine
Depositing User: Mr Tayo Okunlola
Date Deposited: 31 May 2016 12:46
Last Modified: 31 May 2016 12:46
URI: http://eprints.abuad.edu.ng/id/eprint/809

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