CK5/6, CK7 and E-Cadherin; Molecular Differentiation of PCa and BPH Tissue Biopsies

Adegun, P.T and Ogundele, O.M. and Falode, D.T. and Ajonijebu, D.C. and Taiwo, O.J. and Enye, L.A. and Omoaghe, A.O. and Kobomoje, O.S. (2013) CK5/6, CK7 and E-Cadherin; Molecular Differentiation of PCa and BPH Tissue Biopsies. International Journal of Medical Sciences and Biotechnology, I (V). pp. 15-28. ISSN 2321-8509

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Abstract

Background: Two major disorders of the prostate are prostate cancer (PCa) and Benign prostatic hyperplasia (BPH), both of which involve rapid proliferation of cells in the prostate. The clinical manifestations of prostate cancer result from the effects of local growth of the tumor, the spread to regional lymph nodes via the lymphatic system, and the hematogenous dissemination to distant metastatic sites. Although most patients with early-stage prostate cancer are asymptomatic, locally advanced disease can lead to obstructive or irritative voiding symptoms that result from local tumor growth into the urethra or bladder neck, extension into the trigone of the bladder, or both making it difficult to differentiate from symptomatic BPH. However, BPH arises as spherical masses of epithelial and stromal elements from the glands lining the proximal prostatic urethra. The ratio of epithelium to smooth muscle in the prostate can vary among individuals, from 1:3 to 4:1. However, larger prostates may contain more androgendependent epithelial elements than smaller glands, which contain a higher proportion of smooth muscle. In either case, the outcome of BPH may be urethral obstruction induced mechanically by epithelial overgrowth and dynamically by prostatic smooth muscle contraction, or combination of the two. Method: Prostate biopsy samples were collected from the pathology laboratory (4 from patients clinically diagnosed as suffering from BPH and 4 from patients clinically diagnosed with PCa. The specimens were immunohistochemically analyzed using antibodies against E-Cadherin, Cytokeratin 5/6 and Cytokeratin 7 to distinguish cell proliferation of BPH from PCa and down regulation of ECAD. Results and Conclusion: ECAD immunopositivity was greatly reduced in PCa biopsies while over expression were seen in BPH biopsies. Also the CK5/6 was reduced in the PCa biopsies, indicating the tumors are of epithelial origin rather than glandular. CK7 was immunopositive in BPH biopsies indicating that the cells are muscular rather than glandular while CK5/6 was low in BPH biopsies further elucidating the fact that increased population of cells in BPH is not a cancer and is not glandular.

Item Type: Article
Uncontrolled Keywords: Prostate, BPH, CK5/6, CK7, ECAD, cytoskeleton, cell adhesion and epithelium.
Subjects: Q Science > Q Science (General)
Q Science > QD Chemistry
Divisions: Faculty of Engineering, Science and Mathematics > School of Chemistry
Depositing User: Mr Tayo Okunlola
Date Deposited: 07 Jun 2016 11:58
Last Modified: 07 Jun 2016 11:58
URI: http://eprints.abuad.edu.ng/id/eprint/880

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