Immunohistochemical Characterization of Lymphocytic CD3/CD20 and Macrophage CD68 in the Germinal Epithelium of Pb and Se+Zn Treated Adult Sprague-Dawley Rats

Falana, B.A. and Ogundele, O.M. and Duru, F.I. and Osinubi, AA and Falode, DT (2013) Immunohistochemical Characterization of Lymphocytic CD3/CD20 and Macrophage CD68 in the Germinal Epithelium of Pb and Se+Zn Treated Adult Sprague-Dawley Rats. Journal of Cytol. Histol, 4 (171). pp. 1-4. ISSN 2157-7099

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immunohistochemical-characterization-of-lymphocytic-cd3-cd20-and-macrophage-cd68-in-the-germinal-epithelium-of-pb-and-se-zn-treated-adult-sprague-dawley-rats-2157-7099.1000171.pdf

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Abstract

Lead toxicity in the testes has been described to be capable of inducing cell death by apoptosis and necrosis. Such toxicity can be attenuated by selenium and zinc synergy treatment in trace amount. This study evaluates the role and distribution of macrophages/histocytes (CD68), B-Lymphocytes (CD20) and T-Lymphocytes (CD3) in the testes of lead, selenium and zinc treated rats. 60 F1 generation adult male Sprague-Dawley rats were divided into four groups of 15 animals each. Group 1 received normal saline, group 2: 100 mg/Kg of lead acetate, group 3: 100 mg/kg of lead acetate then 2.25 mg/ Kg each of Zinc (Chelated zinc) and Selenium (Sodium Selenium) and group 4: 2.25 mg/kg of zinc and selenium (Se+Zn). The duration of treatment was 56 days following which the animals were sacrificed on the 57th day and the testes harvested and fixed in Bouin’s fluid. CD3, CD20 and CD68 are distributed within the epithelium and the interstitium of the Pb treated testis, the expression level is influenced by the extent of the damage posed by Pb toxicity and not by the proliferative tendencies of Se+Zn treatment did protect the germinal epithelium and the macrophage/lymphocyte cell lines.

Item Type: Article
Uncontrolled Keywords: Selenium; Zinc; Lead; Cell death; Germinal epithelium; Macrophages; B-lymphocytes; T-lymphocytes
Subjects: Q Science > Q Science (General)
Q Science > QD Chemistry
Q Science > QL Zoology
Q Science > QR Microbiology
R Medicine > R Medicine (General)
Divisions: Faculty of Engineering, Science and Mathematics > School of Chemistry
Depositing User: Mr Tayo Okunlola
Date Deposited: 07 Jun 2016 12:17
Last Modified: 07 Jun 2016 12:17
URI: http://eprints.abuad.edu.ng/id/eprint/881

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