Evaluation of the Antimicrobial Potential of Gongronema latifolium Extracts on Some Wound-Associated Pathogens

Enitan, Seyi Samson and Ehizibue, Omodiale Paul and Adejumo, Esther Ngozi and Akele, Yomi Richard and Owolabi, Tolulope Omolara Evaluation of the Antimicrobial Potential of Gongronema latifolium Extracts on Some Wound-Associated Pathogens. Open Science Journal of Pharmacy and Pharmacology, 5 (7). pp. 34-41.

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Abstract

This present study investigated the antimicrobial p otential of the leaf and stem extracts of Gongronema latifolium against some selected wound-associated pathogens: Pseudomonas aeruginosa, Escherichia coli, Proteus mirabilis, Klebsiella pneumonae and Candida albican using the punch-hole agar diffusion method. The ou tcome of the study show that neither the aqueous no r the methanolic leaf and stem extracts of Gongronema latifolium showed any inhibitory activity against the bacteri al and fungal isolates at all the concentrations (200, 150, 100 5 0 and 25 mg/mL) tested either singly or combined; w hereas the positive control was effective against all of them; with zon es diameter of inhibition ranging between 18-24 mm ( Pseudomonas aeruginosa ), 17-23 mm ( Escherichia coli ), 15-23 mm ( Proteus mirabilis ), 18-22 mm ( Staphylococcus auerus ) and 17-24 mm ( C. albicans ). On the other hand, as expected, the negative con trol (distilled water) did not show any zone of inh ibition. The antibacterial activity of the positive control was statistically significant at P value <0.05 when com pared against the extracts and negative control. Although the antimicrobial po tential of extracts of G. latifolium have been previously demonstrated against various clinical isolates, the findings in this current study did not support claims made by d ifferent researchers in previously studies. All the test isolates were foun d to be resistant to the extracts. Lack of inhibito ry activity by extracts of G. latifolium growing in Irolu, Ikenne Local Government Area of O gun state in this present study is plausible and wo uld require further investigation. Due to differences in topogr aphical factors of various geographical locations, nutrient concentrations of the soil, chemical composition, age and time of har vest of the plant, extraction method as well as met hod used for antimicrobial study; it is therefore important that scientific protocols be clearly identified, adequa tely followed and reported.

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