Influence of glyphosate on rhizosphere microorganisms and their ability to solubilise phosphate

AGBOOLA, ADEBANKE ADEORITE and EKUNDAYO, FATUYI OLANIPEKUN and EKUNDAYO, ESTHER AANUOLUWA and FASORO, AYODEJI AKINWANDE and AYANTOLA, KE-HINDE JOSEPH and KAYODE, ADEOYE JOHN (2018) Influence of glyphosate on rhizosphere microorganisms and their ability to solubilise phosphate. JOURNAL OF MICROBIOLOGY AND ANTIMICROBIAL AGENTS. pp. 1-7. ISSN 2396-880X

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Abstract

Objective: To determine cowpea rhizosphere microor-ganisms with phosphate solubilizing potential in a soil treated with glyphosate. Methods: Four different con-centrations of glyphosate herbicides (0.00, 0.50, 1.00, and 3.00) were assayed. Cowpea seedlings were planted and later harvested after 30 days of germina-tion. Microorganisms from rhizosphere treated with glyphosate were cultured and identified using selective media Wakesman A and B agar. The activity of isolates were evaluated by Tricalcium phosphate solubilization and screening for phosphatase in a Pikovskaya me-dium.. Results: Bacillus cereus, Bacillus subtilis, Micrococ-cus luteus, Pseudomonas aeruginosa, Proteus mirabi-lis, Bacillus pumilis, Staphylococcus aureus, Aspergil-lus flavus, Rhizopus nigrifican, and Aspergillus sapro-phyiticus were obtained from the glyphosate treated soil. There was decrease in the bacterial and fungal population in the rhizosphere as glyphosate concentra-tion increased. The concentration of the solubilized Tricalcium phosphate ranged from 42.48 to 515.78gl-1. Micrococcus luteus, Bacillus subtilis, Proteus mirabi-lis, Aspergillus flavus, Aspergillus saprophyticus iso-lated at the different concentrations of glyphosate have increased phosphate solubilization with reduction in their pH. Proteus mirabilis showed the highest phos-phatase activity (23.157mM/min/ml) at 30hrs and As-pergillus saprophyticus showed highest phosphatase activity (35.263 mM/min/ml) at 72hrs. Bacillus sub-tilis had the lowest protein concentration (3.034mg/ml) at 12hrs. Conclusion: This study showed that these rhizosphere microorganisms when applied as inoculants into the soil at different concentration of glyphosate can help increase the availability of soluble phosphates.

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