Ocimum gratissimumLinn. Leaves reducethe key enzymes activities relevant toerectile dysfunction in isolated penile andtesticular tissues of rats

Ojo, Oluwafemi Adeleke and Ojo, Adebola Busola and l Oyinloye, Babatunji Emmanue and Ajiboye, Basiru Olaitan and Anifowose, Omosola Olufisayo and Akawa, Ayodeji and Olaiya, Oluranti Esther and Olasehinde, Oluwaseun Ruth and Kappo, Abidemi Paul Ocimum gratissimumLinn. Leaves reducethe key enzymes activities relevant toerectile dysfunction in isolated penile andtesticular tissues of rats. BMC Complementary and Alternative Medicine.

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Abstract

Background:Ocimum gratissimumL. is a medicinal plant widely grown in tropical and subtropical regions with theleafdecoctionusuallytakeninfolkmedicinetoenhanceerectileperformanceinmenalthoughtheprobablemechanism of actions remains undetermined. This study examined the inhibitory potentials ofOcimum gratissimumleaves on some key enzymes associated with erectile dysfunction in penile and testicular tissues of the rat.Methods:Inhibitory effect of aqueous extract (1:10w/v)ofO. gratissimumleaves on the activities of phosphodiesterase-5(PDE-5), arginase, angiotensin I–converting enzyme (ACE), and acetylcholinesterase (AChE) in penile and testicular tissueswere assessed. Also, the extract was investigated for ferric reducing antioxidant property(FRAP) and 1,1-diphenyl-2-picryl-hydrazil (DPPH) radical scavenging abilities.Results:The extract showed higher PDE-5 (IC50=43.19μg/mL), ACE (IC50= 44.23μg/mL), AChE (IC50= 55.51μg/mL) andarginase (IC50= 46.12μg/mL) inhibitory activity in thepeniletissuethanPDE-5(IC50= 44.67μg/mL), ACE (IC50= 53.99μg/mL), AChE (IC50= 60.03μg/mL) and arginase (IC50=49.12μg/mL) inhibitory activity in the testicular tissue homogenate.Furthermore, the extract scavenged free radicals and in a dose-dependent manner.Conclusion:Theenzymeactivitiesdisplayedmightbeassociatedwiththebioactivecompoundspresentintheextractwhich could possibly explain its use in the management of erectile dysfunction (ED).

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