Effects of Acalypha torta (Muell) Leaf Extract on Histological Indices of the Visceral Organs of Male (Rattus novergicus) Wistar Rats

Odigie, B.E. and Erameh, T.O. and Ekundina, V.O. (2015) Effects of Acalypha torta (Muell) Leaf Extract on Histological Indices of the Visceral Organs of Male (Rattus novergicus) Wistar Rats. IOSR Journal Of Pharmacy, 5 (5). pp. 1-7. ISSN 2250-3013 (online), 2319-4219 (paper)

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Abstract

Acalypha torta (Muell) a lush ornamental plant is commonly used for the treatment of fungal skin diseases, pain, fever, and ulcer. It is used by traditional herbal practitioners in Nigeria without a safe dose regimen. For this reason, the present study examines the histological indices (cyto-architecture) in rats treated orally with A. torta crude leaf aqueous extract. Thirty male Wistar rats with average weight (202.25g) were assigned to 5 groups (n=6). The animals were acclimatized under a standard ambient temperature of 26-28 0C. Food and water were provided as required. Extracts between 50mg/ml to 500mg/ml were administered to the rats in group (A, B, C and D) orally while E served as the untreated group. All rats were sacrificed by (Chloroform) anesthetization at the termination of the experiment. Grossing was done following excision of visceral organs of interest (liver and kidney). Cut tissue at 3-5mm was processed histologically and was sectioned at 3-5microns. Mayer’s haematoxylin and eosin stained sections were examined using the light microscope. The results showed normal histological indices of the liver and kidney examined. Though there were negligible signs of acute histopathology effects but were thought to be dose-related offenses. Therefore, the present study provides support for the safety profile of A. torta herbal preparation in a concentration of 500mg/ml in male Wistar rats. However, further studies may attempt to look at higher doses under similar circumstances.

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