Inhibition of key enzymes linked to type 2 diabetes and sodium nitroprusside-induced lipid peroxidation in rat pancreas by water-extractable phytochemicals from unripe pawpaw fruit (Carica papaya)

Ganiyu, Oboh and Ayodeji, A Olabiyi and Ayodele, J. Akinyemi and Adedayo, O. Ademiluyi (2014) Inhibition of key enzymes linked to type 2 diabetes and sodium nitroprusside-induced lipid peroxidation in rat pancreas by water-extractable phytochemicals from unripe pawpaw fruit (Carica papaya). Antidiabetic potential of different parts of unripe pawpaw fruit in vitro, 25 (1). pp. 21-34.

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Abstract

Background: Various parts of unripe pawpaw (Carica papaya Linn) fruit have been reportedly used for the management or treatment of diabetes mellitus in folklore medicine. Therefore, the present study sought to investigate the inhibitory effects of the aqueous extract of different parts of unripe pawpaw fruit on key enzymes linked to type 2 diabetes (α-amylase and α-glucosidase) and sodium nitroprusside (SNP)-induced lipid peroxidation in rat pancreas in vitro. Methods: The aqueous extracts of the unripe pawpaw (C. papaya) fruit parts were prepared (1:20 w/v) and the ability of the extracts to inhibit α-amylase, α-glucosidase and SNP-induced lipid peroxidation in rat pancreas in vitro was investigated. Results: The results revealed that all the extracts inhibited α-amylase (IC50=0.87–1.11 mg/mL), α-glucosidase (IC50=1.76–2.64 mg/mL) and SNP-induced lipid peroxidation (IC50=1.99–2.42 mg/mL) in a dose-dependent manner. However, combination of the flesh, seed and peel in equal amounts had the highest inhibitory effect on α-amylase and α-glucosidase activities. Conclusions: Strong inhibitory activities of the unripe pawpaw fruit against key enzymes linked to type 2 diabetes and SNP-induced lipid peroxidation in rat pancreas could be part of the mechanism by which unripe pawpaw is used in the management/prevention of diabetes mellitus in folk medicine. However, combining the unripe pawpaw fruit parts in equal amounts exhibited synergistic properties on α-amylase and α-glucosidase inhibitory activities

Item Type: Article
Uncontrolled Keywords: α-amylase; α-glucosidase; diabetes; sodium nitroprusside; unripe pawpaw fruit.
Subjects: R Medicine > R Medicine (General)
S Agriculture > S Agriculture (General)
Divisions: Faculty of Medicine, Health and Life Sciences > School of Medicine
Depositing User: Mr. Victor Sebiotimo
Date Deposited: 13 Mar 2019 11:15
Last Modified: 13 Mar 2019 11:15
URI: http://eprints.abuad.edu.ng/id/eprint/95

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